M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Third Semester
Zoology
Core XIII ANIMAL BIOTECHNOLOGY
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all the questions.
All questions carry equal marks.
1. Write notes on cosmids and plasmids.
2. Write a note on Sasccharomyces cerevisae.
3. Write about the transgenic animals.
4. Gram negative bacteria.
5. DNA finger printing.
6. Define the hybridization sequencing.
7. Write note on stem cell culture.
8. Explain briefly about Vaccine and Humulin.
9. Write notes on cryopreservation.
10. Define the super ovulation.
Sub. Code
509302
RW-862
2
Sp 5
Part B x 5 25)
Answer all the questions, choosing either or
11. Explain the artificial Insemination in cattle.
Or
Explain the principle of recombinant DNA
technology.
12. Discuss about the Knock in and knock out gene
technology.
Or
Explain the tissue plasminogen activator.
13. Write notes on probe labelling and Nick translation.
Or
Explain DNA micro array technology.
14. Explain the PCR based sequencing and
hybridization sequencing.
Or
Explain the E.Coli vectors PBR 322 and its
derivatives.
15. Discuss about the Sanger's dibeoxy sequencing and
pyrosequencing.
Or
Explain the application of genetic engineering.
RW-862
3
Sp 5
Part C x 10 30)
Answer any three questions.
Each questions carry equal marks.
16. Explain the concepts of genetic engineering.
17. Explain the steps involved in gene cloning.
18. Discuss about the nucleic acid hybridization techniques.
19. Discuss about animal tissue and culture methods.
20. Discuss embryo transfer and transgenic animal
technology.
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