M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
First Semester
Zoology
Elective I — ANIMAL CELL CULTURE TECHNOLOGY
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
All questions carry equal marks.
1. Cell culture
2. Organ culture
3. Components of animal cell culture medium
4. Uses of protein free medium
5. Apoptosis
6. Cytotoxicity
7. BCG
8. Stem cells
9. Tissue engineering
10. CEF.
Sub. Code
509501
RW-860
2
Ws15
Part B x 5 25)
Answer all questions by choosing either or
All questions carry equal marks.
11. Explain organotypic culture.
Or
Describe the histotypic culture.
12. Enumerate the role of CO2 in cell culture.
Or
Explain the role of serum and its supplements.
13. Give an account on cell synchronization in animal
cell culture.
Or
How to measure the viability and cytotoxicity?
14. Write short notes on cell cultured based vaccines.
Or
List out the steps involved in the scaling up of
animal cell culture.
15. Write an application of 3D tissue culture
engineering.
Or
Define protein expression in cell culture.
RW-860
3
Ws15
Part C x 10 30)
Answer any three questions.
Each question carry equal marks.
16. Elaborate the structure and types of animal cell culture
techniques with suitable illustrations.
17. Explain the physical, chemical and metabolic functions of
cell culture medium.
18. Write the techniques involved in the long term
mammalian cells in vitro.
19. Detailed account on animal cell culture engineering.
20. Elucidate the applications of the animal cell culture
technology.
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