M.Sc. DEGREE EXAMINATION, NOVEMBER 2017
Third Semester
Botany
PLANT GENETIC ENGINEERING
(CBCS 2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Restriction site.
2. Si nuclease.
3. Cosmid.
4. Ti plasmid.
5. Transposon.
6. Sticky ends.
7. Antisense RNA.
8. X-gal.
9. Plantibodies.
10. Bio safety.
Sub. Code
525505
RW-872
2
ws 4
Part B x 5 25)
Answer all questions choosing either or
11. Describe the types and functions of DNA
polymerase in prokaryotes.
Or
Discuss the mode of action of reverse transcriptase.
12. List out the characteristic features of -phase
vectors.
Or
Comment on shuttle vectors.
13. Discuss any one of the DNA sequencing method in
detail.
Or
Describe briefly the synthesis of cDNA.
14. Describe the types of PCR and its applications.
Or
Comment on applications of DNA finger printing in
plant genetic engineering.
15. Briefly describe the development of golden rice.
Or
Write in short the development of insect resistance
transgenic crop.
RW-872
3
ws 4
Part C x 10 30)
Answer any three questions.
16. Write in detail about the classification and mechanism of
action of restriction endonucleases.
17. Write an essay on the plasmid based vectors for E.coli.
18. How will you construct a gene library? List out its
advantages.
19. Describe the types of blotting techniques used in rDNA
technology.
20. Describe in detail about ethical and environmental
concerns of plant genetic engineering.
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