M.Sc. DEGREE EXAMINATION, APRIL 2017
Second Semester
Microbiology
Core VI — -DNA TECHNOLOGY
(2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. What is phasmids?
2. Merits of CaMV.
3. Define gene cloning.
4. Role of alkali hydrolysis.
5. Importance of Klenow enzyme.
6. What is RFLP?
7. What is somatotropin?
8. Application of gene transformation.
9. What is antisense RNA?
10. Define Electroporation.
Sub. Code
530202
RW-210
2
sp3
Part B x 5 25)
Answer all questions, choosing either or
11. Give a detailed account of DNA modifying enzyme.
Or
Discuss in detail the yeast plasmid vector.
12. Give a brief account of gene cloning.
Or
Explain the role of shot gun approach in developing
gene bank.
13. Explain how DNA sequencing is useful in genetic
engineering.
Or
Define thermal cycler. What is the role of Taq DNA
polymerase.
14. Explain the cloning process for commercial
production of antibiotics.
Or
Write short notes on the melanin biosynthesis in
E. Coli.
15. Give a brief account on the formation of antisense
mRNA.
Or
Write short notes on microinjection method of gene
transfer.
RW-210
3
sp3
Part C x 10 30)
Answer any three of the following.
16. Describe different types of plasmid and phage vectors
used for cloning.
17. Define Human genome project. What are the objectives of
HGP?
18. Define DNA sequencing. Give a detailed account of
Maxam Gilbert method of DNA sequencing.
19. Write an extended note on recombinant vaccine and in
future prospects.
20. Discuss in detail about gene therapy and different
methods in gene therapy.
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