M.Sc. DEGREE EXAMINATION, APRIL 2017
Second Semester
Microbiology
CORE V — MICROBIAL GENETICS
(2016 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Define hot spot in mutation.
2. What is stable gene?
3. Role of DNA photolyase.
4. What is DNA repair?
5. Function of regulator gene.
6. What is represion?
7. Significance of sex factor.
8. Define incompatability.
9. What is sexduction?
10. Sex element.
Sub. Code
530201
RW-209
2
Wk 10
Part B x 5 25)
Answer all questions choosing either or
11. Give a brief account on the origin of spontaneous
mutation.
Or
What is the significance of Luria and Delbruck's
fluctuation test?
12. Explain the mechanism of photo reactivation in
DNA repair system.
Or
Write short notes on SOS repair.
13. Enumerate the salient features of gene concept.
Or
Differentiate between repressors and inducers.
14. Explain the salient features of plasmid.
Or
Explain the structure and replication of plasmid.
15. Explain the conjugation method in prokaryotic cell.
Or
Explain the role of enzyme in the recombination rec
protein.
RW-209
3
Wk 10
Part C x 10 30)
Answer any three of the following.
16. Discuss in detail the molecular mechanism of mutation.
17. Describe the bio chemical mechanism of DNA repair
system.
18. Describe the regulation of gene expression in prokaryote
at the level of DNA.
19. Describe the detection and purification of plasmid DNA.
20. Write an essay on the recombination types.
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