M.Sc. DEGREE EXAMINATION,
NOVEMBER 2017
First Semester
Biochemistry
ENZYME TECHNOLOGY
(CBCS 2014 onwards)
Time 3 Hours Maximum 75 Marks
Part A (10 x 2 20)
Answer all questions.
1. Turnover number
2. Catalytic site
3. Zero order reaction
4. Allosteric site
5. Extracellular enzyme
6. Ion exchange chromatography
7. Bioreactors
8. Encapsulation
9. Artificial enzymes
10. Immuno biosensors.
Sub. Code
4MBC1C3
AFF-4931
2
Wk 3
Part B x 5 25)
Answer all questions.
11. Discuss the multienzyme complex.
Or
List out the common features of enzyme.
12. Exemplify the Irreversible reaction.
Or
Derive LB equation.
13. Give an account on media for enzyme extraction.
Or
Discuss the separation of Intracellular enzyme.
14. Write the advantages and disadvantages of
Immobilization techniques.
Or
Discuss the affinity Immobilization.
15. Illustrate the biosensors.
Or
Give an account on Biochips.
Part C 10 30)
Answer any three questions.
16. Elaborately discuss the Bisubstrate reaction.
17. Discuss any two types of reversible reaction.
AFF-4931
3
Wk 3
18. Illustrate the industrial application of enzymes.
19. Exemplify the immobilization technique.
20. Discuss the ribozymes and abzyme and its application.
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