M.Sc. DEGREE EXAMINATION, APRIL 2018
First Semester
Botany
METHODS IN BOTANY
(CBCS 2017 onwards)
Time 3 Hours Maximum 75 Marks
Section A (10 X 2 20)
Answer all the questions.
1. Quinacrine.
2. Chromosome FISH.
3. Mention any two chemical fixatives.
4. Mordant.
5. Beer Lambert's law.
6. Silica gel.
7. Centrifugal force.
8. Acrylamide
9. Important Value Index.
10. EC.
Sub. Code
7MBO1C2
AFS-0595
2
Ws11
Section B X 5 25)
Answer all the questions, choosing either or
11. Describe the techniques of Fluorescence in in situ
hybridization.
Or
Explain the standard chromosome painting.
12. What is micrometry? How it is useful in
measurement of Cells? Describe the procedure in
detail.
Or
Write notes on Camera lucida with neat diagrams.
13. Explain the principle and functions of UV
Spectrophotometer.
Or
Write short notes on column chromatography.
14. Write briefly on Density Gradient Centrifugation.
Or
Write notes on X-ray diffraction and its
applications.
15. Write an account on methods of data collection and
its importance.
Or
Discuss briefly on microscopic parameters used for
analysis used by you.
AFS-0595
3
Ws11
Section C X 10 30)
Answer any three questions.
16. Describe in detail the precautions needed for a good lab
practices.
17. Explain in detail the methods for specimen preparation of
electron microscopy.
18. Discuss the principle, instrument and applications of
High Performance Liquid Chromatography.
19. Describe the principle and functions of Mass
spectrophotometer.
20. Write a detailed account on importance of morphological
parameters.
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