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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI 600 034
M.Sc. DEGREE EXAMINATION BIOTECHNOLOGY
FIRST SEMESTER NOVEMBER 2017
17PBT1MC04 IMMUNOLOGY
Date: 10-11-2017 Dept. No. Max. 100 Marks
Time: 01:00-04:00
PART A
Answer ALL the Questions
I. Choose the correct answer x 1 5 Marks)
1. Activation of B cell receptor by the binding of an epitope result in the formation of
plasma cells and T cytotoxic cells
memory cells and T cytotoxic cells
Plasma cells for antibody production and memory cells for primary response
Plasma cells for antibody production and memory cells for secondary response
2. MHC alleles are
co-dominant recessive dominant none of the above
3. Type IV hypersensitivity is also referred to hypersensitivity.
IgM mediated IgE mediated IgG mediated delayed type
4. Natural humoral immune response against a pathogen leads to the production of
polyclonal antibodies monoclonal antibodies macrophages CMI
5. In Counter immunoelectrophoresis
electrophoresis will drive the antibody and antigen parallel to each other
electrophoresis will drive the antibody and antigen toward each other
the antibody will migrate towards anode
the antibody will migrate towards cathode
II. State whether the following are true or false. (5x1=5 Marks)
6. IgG can cross placenta and provide immunity to new born.
7. Class III MHC molecules are structurally similar to Class I and II MHC molecules.
8. Herceptin employs humanized monoclonal antibodies directed against the HER2 protein.
9. Oral Polio Vaccine is safer than inactivated Polio Vaccine.
10. Competitive immunoassay can be used to detect small amount of antigen and antibody.
III. Complete the following x 5 Marks)
11. Incomplete antigens are called
12. The Diversity Region is present only in chain of an Immunoglobulin.
13. is a technique to match a patient and a donor for transplantation compatibility.
14. The first vaccine was developed
15. The chemical group on the antigen that can react with the antibody is
IV. Answer the following within 50 words x 1 5 Marks)
16. What are anaphylotoxins?
17. State the chromosomal location of genes coding for human immunoglobulins.
18. Mention one difference between primary and secondary immunodeficiency disorders.
19. What are toxoids?
20. What are immunosensors?
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PART B
Answer the following each within 500 words. x 8 40 marks)
Draw diagrams wherever necessary
21. Classify antigens. Give an account of their properties.
OR
Give a brief account of the the production and function of T lymphocytes
22. Differentiate between class I and class II MHC molecules.
OR
What are the types of organ donors? Add a note on criteria to be considered for
transplantation.
23. What are primary immunodeficiency disorders? Explain the causes, symptoms and treatment of
Adenosine deaminase deficiency.
OR
Comment on the symptoms, induction and treatment of Systemic Lupus Erythematosus.
24. Discuss the function and applications of abzymes.
OR
Explain the production of recombinant vaccines.
25. Briefly explain the principle and applications of Western Blotting.
OR
Discuss the methods available for mapping antibody epitopes on target antigens.
PART C
Answer any TWO of the following, each within 1500 words. x 20 40 Marks)
Draw diagrams wherever necessary.
26. Write in detail on the complement system pathways.
27. Explain the mechanism of site specific recombination within the immunoglobulin gene with reference to
the light chain. Add a note on its significance.
28. Briefly outline the different types of hypersensitivity reactions with examples.
29. Explain the principle, types, procedure and applications of ELISA.