M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Genetics
ANALYTICAL INSTRUMENTS AND TECHNIQUES
Time: 2½ Hours
Max. Marks: 70
Instructions: Section-I is compulsory. From Section-II attempt any four. All questions carry equal marks. Figures to right indicate full marks. Draw neat and labeled diagrams.
Section I
Q.1

Rewrite the following sentences by using correct alternatives:
07
Distance between front surface of the lens and surface specimen is known as
Working Distance
Resolving Distance
Focal length
Focal point
is present at the upper end of body tube.
Nosepiece
Eyepiece
Mechanical stage
Fine adjustment knob
unit used by SI system as unit of radioactivity.
Curie
Millicurie
Becquerel
Femtocurie
Which of the following isotope has very long half life?
15N
20O
14C
36CI
Migration rate under unit potential gradient is known as
Mobility
Absorptivity
Resistivity
Chtomatography
is a stationary phase in paper chromatography.
Ethyl alcohol
Water
Hydrochloric acid
Sodium hydroxide
spectrum of the substance is a fingerprint for its identification.
UV
AAS
IR
VISIBLE

Define the following terms:
07
Microscopy
SDS-PAGE
Becqurel
Stationary phase
Spectrophotometer
Convex lens
Resolving power
Page 2 of 2
SLR-UK-405
Section II
Attempt any four:
Q.2
Explain construction, principle image formation of compound microscope.
14
Q.3
Describe in detail visible spectroscopy.
14
Q.4
Discuss DNA blotting technique.
14
Q.5
Answer any Two of the following:
14

Write a short note on descending chromatographic technique.

Describe proportional counter.

Explain in brief confocal microscope.
Q.6
Answer any Two of the following:
14

Give brief account on LCMS

Write a note on Liquid scintillation counter.

Explain agarose gel electrophoresis.