M.Sc. (Semester IV) (CBCS) Examination Nov/Dec-2018
Nano-Technology
ANIMAL BIOTECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions of Section I are compulsory.
Answer any Four questions from section II.
All questions carry equal marks.
Draw neat and labeled diagrams wherever necessary.
SECTION I
Q.1 Rewrite the sentence after choosing correct answer from the given
alternatives:
07
Transformation of method animal cells in which cells are given shock
treatment is
Micro-injection Lipofection
Electroporation All of these
The production of complete animals from somatic cells of an animal
is called
Cell cloning Animal cloning
Gene cloning All of these
The first successfully cloned animal was
Rabbit Sheep
Rat Dog
The method in which he nucleus of a donor cell is relocated to an
enucleated target cell is known as
Cell transformation Nuclear transplantation
Organ transplant All of these
Sodium bicarbonate is added to cell culture media because
Maintain pH and CO2 concentration
Help adherence of cells to substrate
Control contamination
To promote uptake of CO2 by cells
Which of the following is the easiest contamination to detect
Mycoplasma Yeast
Viral All of these
is used as an indicator of pH in cell culture media.
Methylene blue Eosin
Phenol red Malachite green
Define the following terms. 07
Finite cell lines
Continuous cell lines
Organ culture
Substrate for cell culture
Cell line
Cell senescence
Recombinant protein vaccine
Page 2 of 2
SLR-VT-541
SECTION II
Answer any four of the following:
Q.2 Explain different types of organ culture with suitable diagrams. 14
Q.3 Explain in detail different types of bio-safety cabinets. 14
Q.4 Explain in detail different types of contaminations in cell culture. 14
Q.5 Explain mammalian cloning with 1 example of transgenic animal in detail. 14
Q.6 Answer any Two of the following: 14
Write about natural media used in animal cell culture and its limitations.
Write short notes on chemical sterilization.
Explain any 2 methods of transfection.
Q.7 Write short notes on. (Any Two) 14
Explain the production of monoclonal antibodies with diagram.
Serum Free Media
Explain the importance of carbon dioxide and bi-carbonates in animal
tissue culture media.