M.Sc. (Semester (CBCS) Examination Nov/Dec-2018
Microbiology
MICROBIAL CHEMISTRY AND ENZYMOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions are compulsory.
Figures to the right indicate full marks.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
An enzyme was crystallized for the first time by
John Northrop Edward Buchner
J. B. Sumner Pasteur
Carbohydrates contain carbon hydrogen and oxygen in ratio


One gram of lipids yields Kilo calories heat.
-9.9 +9.9
+9.3 -9.3
Amino acid sequence of insulin, a hormone protein was determined by

Bloor Robert hooke
Frederick sanger Berzelius
Monomeric enzymes contain to amino acid residues.
90 to 100 150 to 100
100 to 300 300 to 400
When fats are added in water they are converted in to droplets and dispersed,
the process is called
Hydrolysis Rancidity
Saponification Emulsification
Glycogen and starch are
Oligosaccharides Disaccharides
Homopolysaccharides Heteropolysaccharides
Vitamin C is called
Folic acid Calciferol
Ascorbic acid Thiamine
Two sugars differ in the configuration around one specific carbon are called

isomers isotopes
epitopes epimers
10) Rickets is caused due to deficiency of
Vitamin-B Vitamin-A
Vitamin-D Vitamin-C
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SLR-VP-428
11) Maltose is made up of and
D-glucose D-galactose D-glucose D-glucose
D-galactose D-galactose D-glucose L-lactose
12) is sugar acid.
Citric acid Succinic acid
Glutamic acid Uronic acid
13) Enzymes of class catalyse the non-hydrolytic cleavage of bonds in
substrate.
Hydrolases Ligases
lyases Transferases
14) Bacteriorhodopsins are present in photosynthetic halobacteria.
Cytoplasm Cell membrane
Cell wall Mesosome
Q.2 Answer any four:-
Define provitamins.
List sulfur containing amino acids.
Rancidity
Group specificity
List aromatic amino acid
08
Answer any two
List different classes of enzymes
Properties of lipids
Bacteriorhodopsin
06
Q.3 Answer. (Any Two)
Write on cytochromes.
Role of orbital steering in catalysis
Basic concepts of cooperatively
08
Answer. (Any One)
MWC and KNF models
Types of lipids and structural aspects
06
Q.4 Answer. (Any Two)
Steroids and their functions
Induced fit hypothesis
Structure of amino acid chemical reactions.
10
Answer. (Any One)
Helix coil transition
Acid-Base catalysis
04
Q.5 Answer. (Any Two) 14
Write on Wilhelmy's and Brown's work.
Describe in detail covalent catalysis.
Nomenclature of fatty acids.