B.Sc. I (Entrepreneurship) (CBCS) (Semester II) Examination, 2018
basic techniques in industrial microbiology
Industrial Microbiology II
Day and Date Friday, 20-4-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
N.B. All questions are compulsory and carry equal marks.
Draw a neat labelled diagram wherever necessary.
Figures to the right indicates full marks.
1. Rewrite the following sentences by choosing correct alternatives from given below 14
is the example of mutagenic agent.
Sodium UV light Visible light Agar
ii) Protoplast fusion technique mainly used in programme.
Strain improvement Screening
DSP Scale up
iii) technique is mainly used in isolation of Auxotropic mutant.
Filtration Penicillin Streak Plate Amino acid
iv) The process adopted to produce an inoculums meeting these criteria is
called as
Hydrolytic enzyme Antibiotic
Scale up Inoculums development
is an industrial important procedure for qualitatively assessing
or quantitatively measuring the amount of fermentation product.
Assay Scale up
Fermentation None of these
vi) Any change that occurs in the DNA of a gene is referred to as
Transformation Vernalisation
Mutation Eugenics
vii) cost is requiring for recovery and purification of ethanol.
30 100 55 70
viii) Ca(OH)2 is mainly used for recovery of
Amylase Penicillin Citric acid Vitamin B12
ix) Data reduction is mainly performed by
Data logging Data recovery
Data analysis Data management
can be precipitated out of broth by the addition of methanol.
Peptone Dextran
NaOH Citric acid
xi) process mainly used in the recovery and purification of alcohols.
Distillation Solvent extraction
Drying None of these
xii) Mutations inducing agents are called as
Toxicants Antifoam agent
Supernatant Mutagens
xiii) Auxotrophic mutants cannot grow on media.
Complete Synthetic
Minimal Semisynthetic
xiv) is mainly used in computer applications in fermentation technology
for ADC to DAC.
Interface Clock Meter Knob
2. Answer any seven of the following 14
Define centrifugation.
ii) Define turbidometric assay.
iii) Enlist types of mutagenic agent.
iv) Applications of drying in DSP.
Define biological assay.
vi) Define Flocculation.
vii) Define downstream processing.
viii) Define scale up.
ix) What is protoplast fusion
3. Answer any two of the following 10
Describe in brief precipitation in DSP.
ii) Discuss in detail genetic engineering in strain improvement
programme.
iii) Describe in detail inoculums development process.
Give a detailed account on Site directed mutagenesis. 4
4. Answer any two of the following 14
Describe in detail fermentation economics.
ii) Describe in detail metabolic response assay.
iii) Explain in detail computer applications in fermentation technology.
5. Answer any two of the following 14
Write in detail solvent extraction for recovery of product.
ii) Discuss in detail enzymatic assay.
iii) Define filtration and explain in detail types filtration.