B.Sc. II (Entrepreneurship) (CGPA) (Semester IV)
Examination, 2018
microbial biotechnology (paper
Molecular Biology
Day and Date Wednesday, 12-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
N.B. All questions are compulsory and carry equal marks.
Draw a neat labelled diagram wherever necessary.
Figures to the right indicate full marks.
1. Rewrite the following sentences by choosing correct alternatives. 14
Topoisomerase is involved in
Producing RNA primer Joining DNA segments
Producing nick in DNA Separation of DNA strands
DNA strand is synthesized in direction.
3
is capable of self replication.
DNA Enzyme RNA Protein
DNA polymerase is
DNA dependent DNA polymerase
RNA dependent RNA polymerase
RNA dependent DNA polymerase
DNA dependent RNA polymerase
is not associated with DNA polymerase I.
to exonuclease activity
to exonuclease activity
to endonuclease activity
to polymerase activity
Set P
is a promoter.
A specific sequence of DNA to which a catabolic repressor binds
A specific sequence of DNA to which RNA polymerase binds
A specific DNA sequence to which a restriction endonuclease binds
None of the above
RNA polymerase in prokaryotes has a removable
Alpha subunit Beta subunit
Delta subunit Sigma subunit
In human, the inherited autosomal recessive diseases Xeroderma
pigmentosum is the result of a defect in the
SOS repair Mismatch repair
Repair in alkylated DNA Repair of UV damaged DNA
In bacterial promoters describe the 'Pribnow box'.
The untranslated region The-10 box
The-35 box The termination sequence
10) is directly reverses DNA damage.
AP endonuclease UVr-ABC
MutS and MutL Methyltransferase
11) Rifampicin is a specific inhibitor of
Bacterial RNA polymerase RNA polymerase III
RNA polymerase I RNA polymerase II
12) During DNA replication in E.coli, RNA primers are synthesized
by
dna B dna C dna G dna A
13) The 20 different amino acids found in proteins are coded by
64 codons 61 codons 60 codons 63 codons
14) enzymes are not used in pyrosequencing.
DNA polymerase Pyrophosphatase
Luciferase ATP sulfurylase
SLR-SK 37 *SLRSK37*
Set P
2. Answer any seven of the following 14
RNA polymerases in Eukaryotes.
Enhancers.
Termination of replication in eukaryotes.
RNA polymerases in prokaryotes.
Topoisomerase.
The Central Dogma.
Pyrosequencing.
RFLP.
RAPD.
3. Answer any two of the following 10
Explain in detail properties of genetic code.
Explain in detail direct repair system.
Describe in brief Sangers Dideoxy method of DNA sequencing.
Write a detail note on enzyme involved in eukaryotic Replication. 4
4. Answer any two of the following 14
Write in detail isolation of Nucleic acid.
Explain in detail Translation in prokaryotes.
Describe in detail Rolling Circle Model.
5. Answer any two of the following 14
Write in detail Lac Operon.
Describe in detail post transcriptional modification.
Explain in detail Mismatch repair.