M.Sc. (Semester IV) (CBCS) Examination Mar/Apr-2018
Zoology
ANIMAL BIOTECHNOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Question number 2 and 6 are compulsory. Attempt any two from Question No. 4 and 5.
Q.1
Tick the correct alternative:- (Per question 2 marks)
14
In the autoclave sterilization is conducted at a pressure of
10 lb
15 lb
12 lb
16 lb
is a technique used in molecular biology research to study gene expression by RNA.
Northern blotting
Southern blotting
Western blotting
All the above
Aminopterin present in the HAT medium inhibits
de novo pathway of nucleotide synthesis
Salvage pathway of nucleotide synthesis
Fatty acid synthesis
Glycolysis and TCA cycle
RNA polymerase III transcribes
Small non coding RNAs
Coding RNAs
Both coding and noncoding RNAs
All the above
is a technique widely used in molecular biology research to detect specific proteins in tissue sample.
Northern blotting
Southern blotting
Western blotting
All the above
DNA sequencing method using the chemical is generally called as method.
Sanger-Coulson
Maxam-Gilbert
Enzymatic
Dideoxy
In eukaryotes, transcription is initiated by
RNA polymerase I
RNA polymerase II
RNA polymerase III
RNA polymerase IV
Q.2
Explain in detail sequencing of nucleic acids and proteins.
14
Q.3
Explain the mechanism of protoplast fusion.
07
Explain hybridoma technology.
07
Q.4
Write note on DNA methylation.
05
Explain mechanism of transposition.
05
Explain renewal of stem-cells by epidermis.
04
Page 2 of 2
SLR-UT-635
Q.5
Explain the cell diversity in early embryo.
07
Explain the principle and methods of gene targeting.
07
Q.6
Write short notes on any four of the following:
14
FISH
Application of genetic engineering in medicine
Totipotency
Ribonucleoprotien
RNA Splicing
Cell lines