B.Sc. (Part (Biotechnology) (Semester II) (CBCS)
Examination, 2018
Taxonomy and Tisue culture (paper iI)
Tissue Culture
Day and Date Wednesday, 18-4-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing most correct alternatives 14
the liquid endosperm from immature coconuts, used as a supplement
in the culture medium.
coconut milk casein juice agar
ii) Reversal of organized structures into an undifferentiated state is
redifferentiation dedifferentiation
organogenesis micropropagation
iii) known as the Father of embryology.
Hamberlandt Murashige
Skoog Maheshwari and Guha
iv) Elements required by plants in higher concentration than 0.5 mmol/liter
referred as
vitamins cofactors
macromolecules micromolecules
Potentiality or property of a cell to produce a whole organism is termed as
apoptosis senescence
totipotancy cell cycle
vi) A type of culture in which cells or cell aggregates are cultured in liquid medium
is called
suspension culture callus
anther protoplast culture
vii) In tissue cultures, a morphogenetic response that results in the production of
new organs, embryos or whole plants from cultured explants or calli derived
from them is called
callogenesis regeneration
micropropagation hardening
viii) An isotonic solution of inorganic salts present in approximately the correct
physiological concentrations used in animal cell culture is
Gamborg solution Balanced salt solution
Serum Plasma
ix) A fragment of tissue transplanted from its original site and maintained in an
artificial medium is called
organ organ system
explant blood
A workstation with filtered air flowing in a laminar (nonturbulent) manner
parallel to or perpendicular to the work surface, to maintain the sterility of
the work is known as
laminar-flow hood growth room
culture room polyhouse
xi) is a culture started from cells, tissues or organs taken directly
from an organism and before the first subculture.
subculture isolation culture
cryopreservation primary culture
xii) Inhibition of plasma membrane ruffling and cell motility when cells are in
complete contact with other adjacent cells is termed as
contact inhibition hybridization
fusion suspension
xiii) Growth of animal cells dissociated from the parent tissue by spontaneous
migration or mechanical or enzymatic dispersal is termed as
cell culture continuous culture
spontaneous culture cancer
xiv) is a determination of living or dead cells, based on a total cell
sample.
cell count cell death apoptosis cell viability

2. Solve any seven of the following 14
What is surface sterilization
ii) How can you develop virus-free plants
iii) What is callus tissue
iv) What do you mean by haploid plants and homozygous lines
Give the role of inverted microscope and micropipette.
vi) What is trypsinization
vii) What do you mean by Anchorage dependence of growth.
viii) Write about continuous cell line
ix) Differentiate between synthetic media and natural animal culture media.
What is osmoticum Give an example of it.
3. Solve any two of the following 10
Plant tissue culture laboratory organization.
ii) Instruments used in Animal tissue culture laboratory.
iii) Rapid clonal propagation (micropropagation).
Explain the concept of cytodifferentiation with respect to callus formation. 4
4. Solve any two of the following 14
Give a detailed account on plant cell suspension culture.
ii) Write a note on protoplast isolation and culture.
iii) Describe animal tissue culture lab organization.
5. Solve any two of the following 14
What is organogenesis Add a note on principles, concept and applications
of somatic embryogenesis.
ii) Discuss animal tissue culture media and add a note on importance of growth
factors of the serum.
iii) What is cell viability Add a note on measurement of cell viability.