B.Sc. III (Semester Biotechnology (New CBCS) Examination, 2018
FERMENTATION TECHNOLOGY
Day and Date Tuesday, 20-11-2018 Total Marks 70
Time 2.30 p.m. to 5.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labeled diagrams wherever necessary.
1. Choose the correct alternative and rewrite the sentences again. 14
organism used in Vitamin B12 production.
Streptomyces griseus
Saccharomyces cerevisiae
Acetobacter suboxydane
Escherichia coli
Corn steep liquor is the waste of industries.
Oil Dairy
Corn None of these
Laboratory scale fermentor having liters capacity.
1 2 1,00,000
100 500 None of these
technique is used for isolation of auxotrophic mutant.
Crowded plate pH
Penicillin Citric acid
The purification and recovery of the production after fermentation is called as process.
Upstream Downstream
Fermentation None of these
Stock culture maintained by
Sterilization Lyophilization
Pasteurization Tyndalisation
Industrial production of citric acid is carried out using microorganism.
Escherichia coli
Aspergillus niger
Saccharomyces cerevisiae
Acetobacter suboxydane
of the following is not used as antifoaming agent.
Citric acid Vegetable oils
Lard oil Silicon derivatives
Solvent extraction is used for recovery of
Penicillin Alcohol
Amylase Citric acid
10) In penicillin G production is the precursor used.
Phenyl acetic acid Alcohol
Vinegar Sodium chloride
11) Fermentation economics is based on
Recovery Incubation period
Medium constituent All of these
*SLRSJ43* SLR-SJ 43
Set P
12) of fermentation process is always done before starting large scale operation of any fermentation.
Scale up Economics
Scale down Screening
13) substrate mainly used in amylase production.
Malt Peptone
Starch Beef extract
14) The sterilization of heat sensitive material in fermentation industry is carried out by
Centrifugation Electrostatic precipitation
Heat Filtration
2. Define and explain (any four) of the following 8
Define fermentation.
Culture Collection Centers for Microorganisms.
Distillation.
Biological assay.
Precipitation.
Write short note on any two of the following 6
Write a note on anaerobic fermentation.
Write a note on filtration.
Write a note on inoculum preparation.
3. Answer any two of the following 8
Write an essay on drying for product recovery.
Explain in detail bioinsecticide production.
Write in detail fermentation economics.
Answer any one of the following 6
Explain in detail primary screening.
Write an essay on amylase fermentation.
4. Answer any two of the following 10
Explain in detail microbial growth kinetics.
Write an essay on penicillin fermentation.
Explain in detail sterilization of fermentation media.
Answer any one of the following 4
Describe in detail batch fermentations.
Give an account on computer applications in fermentation technology.
5. Answer any two of the following 14
Write an essay on Vitamin B12 fermentation.
Write an essay on citric acid production.
Discuss in detail different methods of strain improvement.