B.Sc. II Biotechnology (Semester IV) (CBCS) Examination, 2018
metabolism
Day and Date Thursday, 13-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions carry equal marks.

Figures to the right indicate full marks.
Draw neat and labeled diagrams.
1. Rewrite the following sentences by using correct alternative. 14
Oxidation of malate to oxaloacetate by malate dehydrogenase, requires
ATP NADH FAD NAD
Under aerobic conditions, pyruvate is converted to acetyl COA by
Hexokinase Pyruvate dehydrogenase
Aldosale Phosphoglycerate kinase
Net ATP yield of glycolysis is ATP molecules per molecule of glucose.
Two Three Four Six
In eukaryotes, the citric acid cycle occurs in
Mitochondria Nucleus
Cytosol Endoplasmic reticulum
Which of the following is both ketogenic and glucogenic amino acid
Valine Tryptophan
Lysine Inulin
is an example of uncoupler of ATP synthesis.
4 dinitrophenol DNSA
Tryptomycin IAA
The long chain fatty acyl COA molecules are transported across mitochondrial membrane by conjugating with polar molecule.
Chlorine Citruline
Phenylalanine Carnitine
Set
P
SLR-SJ 32 *SLRSJ32*
Set P
The site for oxidative phosphorylation is of mitochondria.
Outer membrane inner membrane
Matrix Inter membrane space
De Novo purine necleotide synthesis begins with
PRPP PEP GAR FGAR
10) Transamination is the process where
Carboxyl group is transferred from amino acid
Amino acid breakdown takes place
Amino acid synthesis takes place
Amino group is transferred from amino acid
11) In pentose phosphate pathway is the monophosphate sugar used for shunting into other sugars.
Fructose 6 phosphate
Glucose 6 phosphate
Sedoheptulose 7 phosphate
Erythrose 4 phosphate
12) Fatty acids are covalently linked with carrier proteins during their synthesis.
Methyl Ethyl Butyryl Acyl
13) The light reactions of photosynthesis occur in the
Thalakoid membrane Stroma
Epidermis Cuticle
14) The nonprotein amino acid acts as a carrier of amino and carbon atoms during urea cycle.
Aminopteridine Butadiene
Orthine Hypoxanthine
*SLRSJ32* SLR-SJ 32
Set P
2. Answer the following (any 14
Write a note on reciprocal regulation of both glycolysis and gluconeogenesis.
ii) Define uncouplers with one example.
iii) Explain Rubisco's enzymatic activity.
iv) Write the reaction for activity of Glutamate transferase enzyme.
Which irreversible steps of glycolysis are bypassed in gluconeogenesis
vi) Draw the structure of ATP synthase enzyme.
vii) How fatty acids are transported into mitochondria during their breakdown
3. Answer the following (any 10
Explain reactions of non-oxidative phase of pentose phosphate pathway.
ii) Write a note on inhibitors of electron transport chain and ATP synthase complex.
iii) Write a note on location of photosynthesis and photosystem I and II.
Explain sources of atom in purine biosynthesis. 4
4. Answer any two of the following 14
Add an account on biosynthesis of unsaturated of fatty acids.
ii) Write a note on catabolism of amino acids.
iii) Describe 'Calvin Cycle' of photosynthesis.
5. Answer any two of the following 14
Write a note on transfer of electrons in respiratory chain with chemiosmotic coupling hypothesis.
ii) Explain metabolism of glycogen.
iii) Write a note on biosynthesis of pyrimidines.