B.Sc. Biotechnology (Semester II) (CBCS) Examination, 2018
TAXONOMY AND TISSUE CULTURE
Paper II Tissue Culture
Day and Date Saturday, 24-11-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat and labeled diagrams wherever necessary.
1. rewrite the following sentences by choosing correct alternatives. 14
The part of a plant used for culturing is called
Callus Cell Wall
Stock Explant
The fusion of nucleated cell with enucleated cell leads to
form
Chromosome Symmetric Hybrid
Hybrid Cybrid
Stomata were more open in plants grown in presence of higher concentration.
Calcium Potassium
Sodium Magnesium
is the largest organ in human body.
Skin Lungs
Intestine Lungs Heart
Set
P
SLR-SJ 15 *SLRSJ15*
Set P
method is quick and cheap method of cell separation.
Laboratory Physical
Enzymatical Clinical
described procedure to obtain passaged monolayer.
Haberlandt Dulbecco
Carrel Eagle
is a vitamin used in PTC media.
Inositol Cysteine
Biotin Glycine
is a culture without foreign or undesired life forms.
Septic Culture New culture
Axenic Culture Novel culture
is the liquid endosperm from immature coconuts, used as a supplement in the culture medium.
Agar Casein
Juice Coconut Milk
10) Highest concentration of auxin exist at the
Base of any plant organ Leaves
In Xylem Growing tips of plants
11) Most common measurement of viability is based on
Membrane Integrity Dye uptake assay
Dye exclusion assay Metabolic assay
12) cells have finite life span on artificial medium.
Normal Tumor
Cancerous Defected
*SLRSJ15* SLR-SJ 15
Set P
13) Microelements are essential as for many biochemical reactions.
Enzymes Catalysts
Co-factor Nitrogen source
14) Inhibition of plasma membrane ruffling and cell motility when cells are in complete contact with other adjacent cells is termed as
Hybridization Contact Inhibition
Fusion Suspension
2. Answer the following (any seven) 14
Describe in brief gelling agent.
Define continuous cell line.
Wrote a short note on Natural media.
Write a note on growth room in PTC.
Write a note on stage I of micropropagation.
Write a short note on micropipettes.
Write a note on history of animal tissue culture.
Define Totipotency.
Write a note on role of inverted microscope.
3. Answer the following (any two) 10
How will you produce haploid plants by anther culture
Explain cell disaggregation in cold condition by using trypsin.
Describe different methods of isolation of protoplast.
Define cytotoxicity and explain MTT assay. 4
4. Answer the following (any two) 14
Discuss the role of different components of serum.
Explain in detail callus culture.
Describe in detail suspension culture.
5. Answer the following (any two) 14
Define viability and explain membrane integrity and Dye exclusion assay.
Give details of instruments used in ATC laboratory.
Explain laboratory design for plant tissue culture.