B.Sc. I (Semester II) (Biotechnology) Examination, 2018
(CBCS)
environmental pollution and environmental pollution techniques
Paper II microbial techniques
Day and Date Tuesday, 20-11-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to be right indicate full marks.
1. Choose the correct alternative and rewrite the sentences. 14
Mechanism of Gram's staining can be explained by theory.
Lipid content Peptidoglycan
Mg-ribonucleate All of these
II) is technique used for isolation of bacteria.
Serial dilution Inoculation
Incineration Sterilization
III) is example of Neutral stain.
Methylene blue Giemsas stain
Crystal violet Nigrosin
IV) is method used for capsule staining.
Manvel's Grams
Albert's Ziehl-Neelsen
is used as nitrogen source in media preparation.
Agar agar Peptone
Beef extract Yeast extract
VI) Soil is used for preservation
Bacteria Viruses
Fungi Algae
VII) Autoclave is based on principle of
Boiling Dry heat
Disinfection Moist heat
VIII) The organism that uses CO2 as carbon source and light as energy source belongs to
Chemoheterotrophs Photoautotrophs
Photoheterotrophs None of the above
IX) growth is characterized by a double growth cycle consisting of two log phases separated by distinct lag phase.
Diauxic Synchronous
Continuous None of these
Gas pack is used for culturing
Anaerobic bacteria Algae
Fungi Aerobic bacteria
XI) is the most germicidal wavelength of U.V. rays.
150 nm 390 nm
260 nm None of these
XII) Coliform were differentiated from noncoliforms by using medium.
Blood agar Nutrient agar
Eosin methylene Blue agar Azide blood agar
XIII) Serial dilution technique was first introduced by
Robert Koch Alexander Fleming
Joseph Lister Tyndall
XIV) In LTH, pasteurization is achieved at
145°F for 30 min 161°F for 15 sec
300°F for 1 or 2 Sec None of these
2. Answer any seven (out of nine) of the following. 14
Give function of agar and meat extract in microbiological media.
ii) In which media it is used and give its significance-Neutral red
iii) Use of Reducing agents.
iv) Define Lyophilization.
Define Diauxic growth.
vi) Give any two examples of differential media.
vii) Define dye and stain.
viii) Define sterilization.
ix) Give different methods of maintenance of pure culture.
3. Answer any two (out of three) of the following. 10
Explain classification of stains.
ii) Give any two methods used for cultivation of anaerobic bacteria.
iii) Acid fast staining.
Synchronous growth. 4
4. Answer any two of the following. 14
Explain different phases of growth of bacteria.
ii) Give methods of sterilization.
iii) Explain mechanism of Gram staining.
5. Answer any two of the following. 14
Give Nutritional requirements of bacteria.
ii) Give methods of isolation of pure culture of bacteria.
iii) Explain living media and its function.