M.Sc. (Semester III) (CBCS) Examination Nov/Dec-2018
Environmental Science
ENVIRONMENTAL BIOTECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: All questions carry equal marks.
Question No. 1 is compulsory.
Draw neat and labeled diagram wherever necessary.
Q.1 Rewrite the sentences by choosing correct answer from given alternatives: 14
Microbes can be used in
Dye removal BOD removal
Biogas generation All of the above
Which of the following bacteria is called superbug which could clean the oil?
Bacillus subtilus Pseudomonas putida
Pseudomonas denitrificans Bacillus denitrificans
Following is one of the process in bioremediation
Electrophoresis Isolation of plasmid
Phytoremediation All of the above
Nitrate in waste water is responsible for
Acidification Eutrophication
Phosphate solublisation None of these
Bacteria used in Biogas generation are
Halogens Methanogens
Cynobacteria Nitrate fixing bacteria
The widely used staining technique for differentiating bacterial cells depending
upon the colour is
Gram staining Eosin staining
Methylene blue staining None of the above
Barrophilic organisms can tolerate the
High salt concentration High pressure
High sunlight Can sustain in rocks
The process of the concentration of a poison increasing as you move up the
food web is known as
Chemomagnification Diffusion
Bioaccumulation Biomagnifications
In vermin-composting are used to produce compost.
Algae Fungi
Bacteria worms
10) Freshly isolated animal cell cultures are
Primary cultures Secondary cultures
Cell lines None of the above
11) Most commonly used enzyme for enzymatic disaggregation of cell cultures is
Trypsin Endonuclease
Ligase Reverse transcriptase
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SLR-VI-294
12) Pesticides can be estimated by using
Gel Electrophoresis
Atomic Absorption Spectrophotometry
Flame photometry
Gas Chromatography
13) Which gas has a major share in Biogas?
N2 CH4
CO2 O2
14) The process of extracting metals from ore bearing rocks is called as
Bioextraction Microbial extraction
Biofiltration Bioleaching
Q.2 Answer the following any four:-
1. What is Antagonism explain with an example.
2. What is Mycopesticide explain with an example.
3. Define Xenobiotic compounds explain with an example.
4. Define Bioleaching explain with an example.
5. What is Bioaugmentation explain with an example.
08
Write notes on (Any
1. Function of micro-organisms in soil
2. Biofertilizer for biocontrol
3. Superbug for oil degradation
06
Q.3 Answer the following Any
1. Narrate the role of biotechnology for Ex-situ and In situ remediation of
soils.
2. How microorganisms are important for wastewater treatment? Explain
3. What are mycorrhizal fungi? Explain its role in biotechnology?
08
Answer the following any one
1. What are the impacts of spillage of petroleum products in environment?
Also add a note on biodegradation of petroleum products.
2. Explain the environmental significance of algae in ecosystem.
06
Q.4 Answer the following Any
1. Explain the concept of Transgenic plant and its significance.
2. Explain the technique of bio filtration.
3. Explain the concept of microbial nutrition and metabolism.
10
Answer the following any one
1. Explain concept and types of Biological Filtration Processes.
2. Explain concept of Biomethylation and key Biomonitoring process.
04
Q.5 Answer the following. (Any two) 14
What is Phytoremediation? Explain it with reference to soil environment.
Explain the concept of Carbondioxide Sequestration?
What are biopesticides? Explain the benefits of using biopesticides with
suitable example.