M.Sc. DEGREE EXAMINATION, FEBRUARY 2018.
Fourth Semester
Biotechnology
GENETIC ENGINEERING
2 1720442
Time 3 Hours Max. Marks 70
PART — A
Answer any SIX of the following. 5 30 Marks)
All questions carry equal marks.
1. DNA ligases.
2. C-DNA libraries.
3. Immunologic test.
4. Electroporation.
5. Taq promoters.
6. Southern blotting.
7. Identification of genes.
8. PCR.
PART — B
Answer ALL questions. 10 40 Marks)
All questions carry equal marks.
9. Describe the enzymes used in recombinant DNA technology.
Or
Explain reverse transcriptase and their properties and functions.
10. Enumerate the introduction of recombinant DNA molecules in to appropriate
Hosts.
Or
Describe the DNA sequencing by chemical and enzymatic methods.

11. Explain the expression of proteins with His taq and its significance in
simultaneous expression.
Or
Write an account on DNA finger Printing and its significance.
12. Describe the DNA micro array technology and its significance.
Or
Explain NGS technologies, CRISPR-Cas 9 technology for genome editing.
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