M.Sc. DEGREE EXAMINATION, FEBRUARY 2018.
Fourth Semester
Biochemistry
Paper I RECOMBINANT DNA BIOTECHNOLOGY
2 1720341
Time 3 Hours Max. Marks 70
PART — A
Answer any SIX of the following. 5 30 Marks)
All questions carry equal marks.
1. Nucleases
2. Topoisomerases
3. RFLP
4. Taq promoters
5. Alkaptonuria
6. Haemophilia
7. Embryo transfer
8. Fertilization of oocytes
PART — B
Answer ALL questions. 10 40 Marks)
All questions carry equal marks.
9. Describe the enzymes used in r-DNA technology.
Or
Write an account on cloning vectors.
10. Enumerate the DNA finger prining.
Or
Describe introduction of recombinant DNA molecules in to appropriate Hosts.

11. Explain the applications of transgenic animals and Biopharming.
Or
Write an account on the production of growth hormones and their uses.
12. Describe the creation of Dolly and nuclear programming.
Or
Explain the methods of stem cells isolation and culture.
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