DISTANCE EDUCATION
M.Sc. (Botany) DEGREE EXAMINATION, MAY 2018.
Specialization Plant Biotechnology
PLANT GENETIC ENGINEERING
(2008 Onwards)
Time Three hours Maximum 100 marks
Answer any FIVE questions.
All questions carry equal marks.
x 20 100)
1. Discuss plant genome project citing suitable
example.
Explain the protocol for agarose gel electrophoresis.

Describe southern blotting technique.
2. Give an account on application of plant genetic
engineering.
Describe the different types of restriction and
modification systems recognized in gene
manipulation.
Present the importance of choice of markers on
cloning vectors.
3. Write about the promoters used in plant vectors.
Describe the types of reporter genes and their role
in optimizing transformation.
Enumerate the desirable features of plasmid cloning
vehicle citing a suitable example.
Sub. Code
25
DE-4084
2
Sp 6
4. Evaluate the importance of plant tissue culture for
most transformation procedures of plants.
Describe the Agnobacterium mediated
transformation.
Present different selectable markers for plants.
5. Write an account on gene silencing.
Discuss the role of organelle genome in cytoplasmic
male sterility.
Elucidate the properties and function of cross
protection satellite RNA.
6. Explain how plant genetic engineering helpful in
production of abiotic stress tolerant varieties.
Describe the genetic manipulation strategy to delay
ripening of fruit.
Write a detailed account on Bt crops.
7. Discuss various direct plant transformation
techniques.
Explain the salient features and advantages of
binary vectors with examples.
Write short note on micro satellite.
8. Write an account on golden rice.
Present the structure and significance of
microsatellites.
Discuss how resistance to fungal pathogen is
achieved by gene manipulation.