Answer any three questions. Each carries 10 marks.

1. Discuss different methods available for decalcification. Mention advantages and disadvantages of each method.

2. Describe the procedure for embedding of tissue. Write in brief paraffin block preparation.

3. Mention the various stains used in cytology and write about Papanicolaou stain (PAP).

4. Define and classify fixative. Describe compound fixatives.

5. Write short notes on any four of the following:

Clearing

Reception of specimen

Cytological fixative

Cryostate technique

Advantages and disadvantages of cytopathology

6. Write briefly on any two of the following:

Microtomes (types and uses)

Honing

Serial sections

7. Indicate True or False for the following:

The general term used for the removal of tissue from dead body is biopsy.

The melting point of paraffin wax is 70°C.

The ideal thickness of histopathology section for microscopy is 5 microns.

Stain used for cervical cytology is PAP.

Alcohol is dehydrating agent.

8. Fill in the blanks.

Eosin stain is best considered as

CO2 gas is used to Freeze section.

Neutralisation of the tissue after acid treatment with alkali is termed as

The amount of fixative in volume in elevation of tissue size is

The process of sharpening the knife edge is