M.Sc. (Bioinformatics) (Semester II) (CBCS) Examination, 2017
MICROBIOLOGY AND BIOTECHNOLOGY
Day Date: Friday, 21-04-2017 Max. Marks: 70
Time: 10.30 AM to 01.00 PM
Instruction
Part-I, Questions-1 is compulsory.
Attempt any four question from part- II.
Figures to the right indicate full marks.
Answer to the Part- I and Part- II are to be written in same
answer Booklet only.
Section-1
Q.1 Rewrite the sentence after choosing the correct answer from the
given alternatives:
07
The bacterium grows in salt concentration is called
Acidophiles Alkaliphiles Halophiles Xerophiles
bacterial genome was synthesized artificially.
Mycoplasma E. coli Pseudomonas H1N1
Sanger's method of sequencing makes use of
Oligonucleotide Deoxynucleotide
Dideoxynucleotide All of these
Molecular taxonomy is based on
28S rRNA 16S rRNA 30S rRNA 23S rRNA
The genetic material of virus is
RNA DNA RNA or DNA Protein
Prions are basically made up of
RNA Protein DNA Virus
plasmid contains viral fragment.
Phagemid cosmid pBR322 pUC19
Definitions: 0
Ribosomes
Endonuclease 07
SV40
BACs
Page 2 of 2
Probes
Domain of life
Growth kinetics
Section- II
Answer Any Four of the following:
Q.2 Explain the general structure of prokaryotic cell with neat labeled diagram. 14
Q.3 Describe the structure and function of cloning vectors-pUC18 and pBR322. 14
Q.4 Explain the gene transfer in plant systems. 14
Q.5 Answer any two from the following: 14
Add a note on application of r-DAN technology in crop improvement.
Write a note on animal tissue culture media.
Explain the stages of growth curve.
Q.6 Write short notes on (any two) 14
Electroporation
Structure of T4 bacteriophage
Bacterial conjugation