M.Sc. (Semester II) (CBCS) Examination Mar/Apr-2018
Bioinformatics
MICROBIOLOGY AND BIOTECHNOLOGY
Time: 2½ Hours
Max. Marks: 70
Instructions: Section-I, Question 1 is compulsory. Attempt any four questions from Section-II. Figures to the right indicate full marks. Answer to the Section-I and Section-II are to be written in same answer Booklet only.
Section I
Q.1

Rewrite the sentence after choosing the correct answer from the given alternatives:
07
belongs to three domains of life.
Archaea
Bacteria
Eukarya
All of these
In process the genetic material is transferred from donor to recipient cell through virus.
Transduction
Conjugation
Transformation
None of these
In phase the growth rate is equal to death rate.
Lag
Log
Stationary
Decline
Sterilization by UV light is called
Wet
Chemical
Physical
None of these
DNA is transferred into the cells that produce reproductive cells, eggs or sperm, in the body is called gene therapy.
Somatic
Germ line
Both a and b
None of these
classified viruses based on genetic material.
Baltimore
Watson
Ion Wilmut
None
Radioactive compounds are used in
Primers
Probes
Plasmids
All of these

Definitions.
07
Totipotency
Endospore
16S rRNA
MC Site
T4 Bacteriopage
SCO
ddNTPs
Section II
Answer any four of the following.
Q.2
Describe the single cell protein and single cell oil. Add a note their applications.
14
Q.3
Explain bacterial artificial chromosomes and yeast artificial chromosomes.
14
Q.4
Explain the techniques for isolation of microorganisms.
14
Q.5
Answer any two of the following.
14
Applications of recombinant DNA Technology.
Structural staining in bacteria.
Growth kinetics.
Q.6
Write short note any two of the following.
14
Mycoplasma
Different media used for plant tissue culture
Bacterial transduction