B.Sc. II (Biotechnology) (Semester IV) (CGPA) Examination, 2018
molecular biology i
Day and Date Thursday, 3-5-2018 Max. Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labelled diagram wherever necessary.
1. Choose and write correct answers from given four alternatives 14
In Blenders experiment the phage protein was labelled with the help of
32 PO4 35SO4 T2 15N
Bent DNA structure can be produced by antitumor drug
Ciprofloxacin Novobiocin
Nalidixic acid Cisplastin
In Z DNA number of base pairs per complete turn is
10 11 12 None of these
Arthur Korenberg indentified the enzyme involved in replication
DNA polymerase III DNA polymerase I
DNA ligase DNA primase
The number of base pairs of DNA in E. coli origin is
245bp 220bp 232bp 241bp
The length of the DNA replicated in 30 minutes in E. coli is
10 mm 1 mm 100 mm 1000 mm
Okazaki fragments are joined together into a continuous strand by the
activity of the enzyme
DNA polymerase DNA polymerase II
DNA polymerase III DNA ligase
The SOS repair mechanism is activated by
5-Bromouracil Hydroxylamine
Acridine orange Thymidine dimers
M. Messelson and F. W. Stahl verified semi conservation nature of DNA
replication by using
Autoradiography Fluroscent label
Electron microscopy Isotopic labelling
10) The mode of replication which resemble the Greek Letter sigma is
Replicon model D-Loop model
Rolling circle model Replication fork model
11) Enzyme called the molecular glue is
DNA Polymerase DNA Ligase
DNA Helicase DNA Primase
12) The codon referred to as Amber is
UAG UGA AUG AUA
13) The protein present in Eukaryotic DNA is
Arginine Lysine Methionine Histone
14) DNA repair mechanism and application was studied by
James Watson Francis Crick
Thomas Lindahl Arther Korenberg
2. Solve any seven of the following 14
Define Gene.
Define Denaturation.
Define linking number.
Define supercoiling.
Define Cot curves.
Define Chargaff's Rule.
Define Coliphages.
Define Wobble hypothesis.
Justify whether the nucleic acid is DNA or RNA.
A 33% G 17% T C 17% U 33%
3. Attempt any two of the following 10
Write in detail Hershey and Chase experiment with a neat labelled
diagram.
Write in detail about the DNA structure with a neat labelled diagram.
Explain Cot curve analysis with a neat labelled diagram.
Explain about the properties of genetic code. 4
4. Attempt any two of the following 14
Explain in detail the enzymes involved in DNA replication of prokaryotes.
Explain mitochondrial and chloroplast genome in detail.
Explain the organisation of genome in eukaryotes with a neat labelled
diagram.
5. Attempt any two of the following 14
Write in detail nucleotide excision repair and SOS repair mechanism.
Explain the process of replication of DNA in eukaryotes with a neat labelled
diagram.
Write in detail about D-Loop model of replication with a neat labelled
diagram.