B.Sc. II (Biotechnology) (Semester IV) (CGPA) Examination, 2018
molecular biology i
Day and Date Friday, 7-12-2018 Total Marks 70
Time 10.30 a.m. to 1.00 p.m.
Instructions All questions are compulsory.
Figures to the right indicate full marks.
Draw neat labelled diagram wherever necessary.
1. Choose and write correct answer from given four alternatives. 14
demonstrated that genes are made up of DNA.
Miescher Avery
Pauling Martha Chase
Nucleic acids are mainly found in
Only nucleus Only cytoplasm
Both nucleus and cytoplasm None of these
The total number of termination codons present in genetic code is
2 3 4 1
The diameter of DNA molecule is
20A° 10A° 30A° 3.4A°
Bent DNA structure can be produced by antitumor drug
Ciprofloxacin Novobiocin
Nalidixic Acid Cisplastin
The smallest chloroplast genome is found in
Red algae Pine
Tobacco Tomato
Set
P
Triplet code phenomenon was observed by F.H.C. crick by using dye.
Acridine Feuelgen Acetocarmine Evan's Blue
In photoreactivation the enzyme photolyase dimers.
G-G C-C T-T A-A
percent of Arginine and Lysine makes histone proteins basic.

10) enzyme catalyse the formation of negative super coils.
DNA polymerase DNA Helicase
DNA ligase DNA Gyrase
11) Mitochondrial DNA mutation leads to the decline of
Glycolysis Photosynthesis
Gluconeogenesis Oxidative phosphorylation
12) Genes essential for all cell types are called
Xist Gene Luxury gene
House Keeping Genes Slave gene
13) Rolling circle model of replication occurs in the E.coli chromosome during
Transformation Conjugation
Transduction Replication
14) The antibiotic ciprofloxacin inhibits enzyme.
Bacterial gyrase Bacterial polymerase
Bacterial ligase Bacterial Helicase
2. Solve any seven of the following 14
Define Tetranucleotide Hypothesis.
Define Bacteriophages.
Define Solenoid.
Define Chargaff's Rule.
SLR-SJ 33 *SLRSJ33*
Set P
Define Gene.
Define Central dogma.
Define Gene deserts.
Define Linking Number.
Define DNA Damage.
3. Attempt any two of the following 10
Explain about the molecular nature of Gene.
Write about the salient features of double Helix of DNA with a neat labelled diagram.
Write about Meselson and Stahl's Experiment of DNA Replication with neat labelled diagram.
Explain in detail about Cot curve Analysis with neat labelled diagram. 4
4. Attempt any two of the following 14
Describe in brief the organisation of DNA in Eukaryotes with a neat labelled diagram.
Write in detail about the properties of Genetic Code.
Describe in brief the Replication of DNA in prokaryotes with a neat and labelled diagram.
5. Attempt any two of the following 14
Describe mismatch and Excision repair mechanism of DNA with a neat labelled diagram.
Describe in detail DNA replication process in Eukaryotes with a neat labelled diagram.
Write in detail about D-loop model of replication in mitochondria with a neat labelled diagram.