M.Sc. (Semester II) (CBCS) Examination Nov/Dec-2018
Bioinformatics
MICROBIOLOGY AND BIOTECHNOLOGY
Time: 2½ Hours Max. Marks: 70
Instructions: Part-1, Question 1 is compulsory.
Attempt any four questions from Part-II.
Figures to the right indicate full marks.
Answer to the Part-I and Part-II are to be written in same answer Booklet
only.
Section I
Q.1 Rewrite the sentence after choosing the correct answer from the
given alternatives:
07
The concept of totipotency was proposed by
Haberlandt Skoog
Cocking Miller
The genome size of mycoplasma is
1.5 2.5mbps 1.7 2.3mbps
2.0 2.2mbps 0.5 1.3 mbps
Hormone used to induce shoots in plant.
Gibberellins Cytokines
Both a b None
Malachite green is used in structural staining.
Cell wall Capsule
Endospore Falgella
LacZ gene is present in plasmid.
pUC19 pBR322
pUC18 Both a c
Serum media is used in
Plant cell culture Animal cell culture
Bacterial None
Molecular taxonomy is based on
28S rRNA 16S rRNA
30S rRNA None of these
Definitions: 07
DNA vaccine
HindIII
Bacteriophage
Nucleoid
ICSB
Clustal W
Lytic cycle
Page 2 of 2
SLR- VC-53
Section II
Answer any four of the following.
Q2 Explain general characteristics and classification of plant viruses. 14
Q3 Explain the different artificial media used in animal cell culture media. 14
Q4 Explain the technique of Sanger's method of DNA sequencing 14
Q5 Answer any two of the following. 14
Explain the different staining techniques.
Molecular genetic analysis of human diseases.
Write a note on bacterial Transformation.
Q6 Write short notes any two of the following. 14
GMOs advantages and disadvantages.
Gene therapy
Phylogenetic tree