B.Sc. (Three Year) DEGREE EXAMINATION, MARCH/APRIL 2017.
End Semester Examination
Second Semester
Part II Biochemistry
NUCLEIC ACID AND BIOCHEMICAL TECHNIQUES
2 C 2301
Time 3 Hours Max. Marks 70
PART — A
Answer any FIVE of the following questions. 4 20 Marks)
1. Structure of nucleic acid.
2. Porphobilinogen properties.
3. Paper chromatography.
4. Use of radioactive isotopes in biology.
5. Tissue slice technique.
6. Cot curve and their significance.
7. Heme.
8. SDS PAGE.
PART — B
Answer ALL the following questions. 10 50 Marks)
9. Discuss about Watson and Crick model of DNA.
Or
What is denaturation and renaturation of DNA? Discuss about Tm values
and their significance.
10. Define porphrin. Write about properties and identification of porphyrin.
Or
Discuss about structure of metalloporphyrin heme, cytochrome and
chlorophylls.

11. Write a principle and applications of centrifugation techniques.
Or
Write a principle and applications of thin layer chromatography.
12. Define Beer-Lambert law. Discuss about a principle and applications of
UV-visible spectroscopy.
Or
Write an account on principle and applications of fluorimetry.
13. Discuss about intermediary metabolism in vivo studies of removal of organs
and perfusion studies.
Or
What is intermediary metabolism? Discuss in a brief about methods of
investigation of intermediary metabolism.